Separated from the analyte or other components contained in the matrix. High purity, not being present in the sample matrix, and being well
Requires similar physical and chemical properties to the analyte, Of the internal standard acetone can improve recovery and precisionĭue to the analyte loss during the testing process. In this development of the formaldehyde analysis method, the use Gas chromatography-mass spectrometry instrument with static headspaceĪs an automatic sampler can also increase the number of samples thatĭerivatization reaction of formaldehyde with p-toluenesulfonic acid in ethanol. Is a modern instrument with high sensitivity and involving simpleĪnalysis techniques for quantifying a wide range of molecules and
Method is expected to be applied in many laboratories because GC-MS Other components commonly found in cream cosmetic products. Instrument of gas chromatography-mass spectrometry (GC-MS) that hasīeen previously developed by taking into account the influence of Of this study is to modify the analysis method by the The availability of the reagent compared to DNPH. Such as the validated method, efficiency in time, and, most importantly, Its several advantages can be applied in the laboratories of Indonesia, The reaction of formaldehyde and ethanol as solvent with an acid catalyst. An alternative derivatization using paratoluenesulfonicĪcid is proposed to obtain volatile diethoxymethane as a product of However, thisĭNPH reagent is currently difficult to obtain due to the Indonesia Of formaldehyde released from the formaldehyde releaser class of preservativesĭerivatization using 2,4-dinitrophenylhydrazineĬommonly used method for formaldehyde determination. (GC-MS) and flame ionization detection (GC-FID) determine the levels
5 However, further modification is needed to update (DNPH), 1 and GC-MS derivatization of DNPH. Methods of formaldehyde have been developed betweenĢ0, including the DLLME-LC UV–vis method, 4 high-performance liquid chromatography-diodeĪrray detector (HPLC-DAD) derivatization of 2,4-dinitrophenylhydrazine This method should have the characteristics for identifyingĪnd quantitatively determining the levels of formaldehyde releasedįrom the preservatives used in cream cosmetics.
These products using the latest, sensitive, and validated analytical 3 Therefore, to meet the safety, efficacy, and quality requirementsįor cosmetics, notably formaldehyde releasers, it is crucial to analyze On Cancer (IARC) in 2006, formaldehyde was classified into group IĪs a human carcinogen based on evidence that formaldehyde substanceĬan cause nasopharyngeal cancer and myeloid leukemia. 2 According to the International Agency for Research Potentially cause allergies (allergens) when used in excess. Used in cleansing and skincare cosmetics products, they also might 1 However, despite these components being commonly Urea (IU) are the most widely used preservatives in cosmetics. Requirements for a validated analytical method and could be appliedįormaldehyde releasers such as diazolidinyl (for PTS liquid by GC-FID), respectively. Interval 95.0–105.0%, a limit of detection and quantitation The validated method showed intradayĪnd interday precision, an accuracy (% RSD) of less than 3.7%, confidence (DNPH) method with high-performance liquid chromatography (HPLC) toĬompare the quantification results. In addition, sample analysis was also carried out using the 2,4-dinitrophenylhydrazine Time and temperature of the reaction to yield the highest peak area. Of p-toluenesulfonic (PTS) acid in ethanol (acidifiedĮthanol), used as the derivatization agent, and the conditions of
Modifications involved optimizations of five series of concentrations Therefore, this study aimed toĭevelop a new method using gas chromatography-mass spectrometry (GC-MS)Īnd gas chromatography-flame ionization detection (GC-FID). The problems involved in the standard method is that of obtainingĪ derivatization agent, especially for routine analysis in the NationalĪgency of Drug and Food, Indonesia. Have developed various methods for determining formaldehyde. However, formaldehyde releaser substancesĪre still widely used as a preservative in cosmetics. Formaldehyde has been reported to be a potential humanĬarcinogen due to its toxicity.